Comparision of chemiluminescence and ELISA kits for SARS-CoV-2 IgM/IgG detection

Objective Chemiluminescence and enzyme-linked immunoassay (ELISA) were used to rapidly detect the immunoglobulin M (IgM) and immunoglobulin G (IgG) of the new coronavirus (SARS-CoV-2) and the results of the two methods were compared . Methods Chemiluminescence and ELISA analysis technology were used to detect the serum SARS-CoV-2 antibody IgM/IgG in 97 patients with new coronavirus pneumonia (COVID-19) nucleic acid, and these two detection methods were used to detect other non-SARS-CoV-2 100 samples of infected serum. Results The sensitivity and specificity of the detection results of SARS-CoV-2 IgM chemiluminescence detection kit were 61.9% (60/97) and 100% (100/100), respectively. The sensitivity of this method for the detection of SARS-CoV-2 IgG And specificity are 92.8% (90/97) and 100% (100/100) respectively; the sensitivity and specificity of SARS-CoV-2 IgM ELISA test kit are 3.1% (3/97) and 100% respectively (100/100); the sensitivity and specificity of this method for SARS-CoV-2 IgG detection were 62.9% (61/97) and 100% (100/100), respectively. Conclusion In this experiment, the sensitivity of chemiluminescence and ELISA detection methods for SARS-CoV-2 IgG detection was higher than that of SARS-CoV-2 IgM, and the sensitivity of chemiluminescence detection was higher than that of ELISA method.